Journal: bioRxiv

Article Title: Decoding scalp health and microbiome dysbiosis in dandruff

doi: 10.1101/2024.05.02.592279

Figure Lengend Snippet: Relative abundance of the key genera, Cutibacterium (C), Staphylococcus (S), and Malassezia (M), and their member species of the scalp and follicle microbiomes collected from healthy and DSD volunteers (A). Correlation analysis (Pearson’s correlation coefficient, PCC) of the relative abundance of the microbiome members between the scalp and follicle of each volunteer in both Healthy and DSD groups at genus (B) and Species (C) level. The blue line indicates the PCC cut-off for over 75% of the individuals compared. Correlation of relative abundance between scalp and follicle of C. acnes (D), M. restricta (E), and S. capitis (F).

Article Snippet: Unassembled sequencing reads were directly analysed by CosmosID-HUB multi-kingdom microbiome analysis Platform (CosmosID Inc., Germantown, MD, USA) for taxonomic profiling of the microbes.

Techniques:

Journal: bioRxiv

Article Title: Decoding scalp health and microbiome dysbiosis in dandruff

doi: 10.1101/2024.05.02.592279

Figure Lengend Snippet: Change in total dandruff score (A), and M. restricta numbers in relation to C. acnes (B) after 28 days of application of formulation with and without propionate. Schematic representing the mechanisms by which the members of healthy and DSD follicle-scalp microbiome interact and impact the host health (C). * P< 0.05.

Article Snippet: Unassembled sequencing reads were directly analysed by CosmosID-HUB multi-kingdom microbiome analysis Platform (CosmosID Inc., Germantown, MD, USA) for taxonomic profiling of the microbes.

Techniques: Formulation

Journal: Foods

Article Title: In Vitro Human Gastrointestinal Digestibility and Colonic Fermentation of Wheat Sourdough and Yeast Breads

doi: 10.3390/foods13183014

Figure Lengend Snippet: Microbiota metabolic activity in the Colon-on-a-plate™ model (undigested fraction) ( a ) total SCFA, ( b ) acetate, propionate, butyrate, ( c ) lactate, ( d ) total BCFA. Data from each of eight healthy donors were considered data replicates. Error bars represent standard deviation ( n = 8 donors). Paired two-sided t -tests were used to determine significant differences between each condition versus the blank when accounting for the entire 48 h period. * p < 0.05. BCFA = branched chain fatty acid; BR = bread recipe; SCFA = short-chain fatty acid.

Article Snippet: The taxonomic classification of the unassembled sequencing reads was analyzed directly using the CosmosID-HUB Microbiome Platform (CosmosID Inc., Germantown, MD, USA) for multi-kingdom microbiome analysis and quantification of relative abundances, as previously described [ , , , ].

Techniques: Activity Assay, Standard Deviation

Journal: Foods

Article Title: In Vitro Human Gastrointestinal Digestibility and Colonic Fermentation of Wheat Sourdough and Yeast Breads

doi: 10.3390/foods13183014

Figure Lengend Snippet: Analysis of the effects of the undigested fractions on microbiota ( a ) alpha (Chao1 and Shannon diversity indices) and ( b ) beta diversity (Principal Component Analysis) in the Colon-on-a-plate™ model. For a, error bars represent standard deviation ( n = 8 donors). For b, this first plane displays 35% of the total variance (respectively, 20% for the first axis and 15% for the second axis). BR = bread recipe.

Article Snippet: The taxonomic classification of the unassembled sequencing reads was analyzed directly using the CosmosID-HUB Microbiome Platform (CosmosID Inc., Germantown, MD, USA) for multi-kingdom microbiome analysis and quantification of relative abundances, as previously described [ , , , ].

Techniques: Standard Deviation

Journal: Foods

Article Title: In Vitro Human Gastrointestinal Digestibility and Colonic Fermentation of Wheat Sourdough and Yeast Breads

doi: 10.3390/foods13183014

Figure Lengend Snippet: Differential abundance analysis (LEfSe) to identify differences in microbiota community composition (species level) at 24 h after the start of incubation with ( a ) undigested BR1, ( b ) undigested BR2, ( c ) undigested BR3, or ( d ) undigested BR4 versus blank. The analyses are based on relative abundance data (total sum scaling). The LEfSe bar plot shows significantly altered bacterial species between the blank and the indicated test product. Sections highlighted in yellow represent features that were significantly enriched by the undigested BR, while sections in purple were more abundant in the blank. The x -axis represents the LDA score (measure of effect size), with LDA scores of ±2 generally accepted as biologically relevant.

Article Snippet: The taxonomic classification of the unassembled sequencing reads was analyzed directly using the CosmosID-HUB Microbiome Platform (CosmosID Inc., Germantown, MD, USA) for multi-kingdom microbiome analysis and quantification of relative abundances, as previously described [ , , , ].

Techniques: Incubation

Journal: Foods

Article Title: In Vitro Human Gastrointestinal Digestibility and Colonic Fermentation of Wheat Sourdough and Yeast Breads

doi: 10.3390/foods13183014

Figure Lengend Snippet: Differential abundance analysis (treeclimbR) to identify differences in microbiota community composition at 24 h after the start of incubation with undigested BR1, undigested BR2, undigested BR3, or undigested BR4 versus blank. The analysis is based on relative abundance data (total sum scaling). The scatter plot classifies taxa into four categories based on abundances in the compared conditions: neither biologically nor statistically significant (gray), biologically significant but not statistically significant (teal), statistically significant but not biologically significant (blue), or biologically and statistically significant (red).

Article Snippet: The taxonomic classification of the unassembled sequencing reads was analyzed directly using the CosmosID-HUB Microbiome Platform (CosmosID Inc., Germantown, MD, USA) for multi-kingdom microbiome analysis and quantification of relative abundances, as previously described [ , , , ].

Techniques: Incubation

Journal: Foods

Article Title: In Vitro Human Gastrointestinal Digestibility and Colonic Fermentation of Wheat Sourdough and Yeast Breads

doi: 10.3390/foods13183014

Figure Lengend Snippet: Differential abundance analysis (LEfSe) to identify differences in microbiota community composition (genus level) at 24 h after the start of incubation with ( a ) undigested BR1, ( b ) undigested BR2, ( c ) undigested BR3, or ( d ) undigested BR4 versus blank. The analyses are based on relative abundance data (total sum scaling). The LEfSe bar plot shows significantly altered bacterial genera between the blank and the indicated test product. Sections highlighted in yellow represent features that were significantly enriched by the undigested BR, while sections in purple were more abundant in the blank. The x -axis represents the LDA score (measure of effect size), with LDA scores of ±2 generally accepted as biologically relevant.

Article Snippet: The taxonomic classification of the unassembled sequencing reads was analyzed directly using the CosmosID-HUB Microbiome Platform (CosmosID Inc., Germantown, MD, USA) for multi-kingdom microbiome analysis and quantification of relative abundances, as previously described [ , , , ].

Techniques: Incubation

Journal: mHealth

Article Title: A pilot evaluation of Swasthya Pahal program using SMAART informatics framework to support NCD self-management

doi: 10.21037/mhealth-20-110

Figure Lengend Snippet: Information flow in Swasthya Pahal (health for all) implementation using SMAART informatics platform.

Article Snippet: Swasthya Pahal (health for all) using SMAART informatics platform Swasthya Pahal (health for all) is an innovative community-based program, facilitated through an interactive, multi-lingual, standalone, and internet-enabled touch screen computer-based program that aims to enhance self-management of NCDs including diabetes, hypertension, and obesity among individuals living across diverse community settings.

Techniques: